They are supplied in a loading buffer for their direct loading in gel lanes. Most commercial prestained protein ladders are available as three-color protein standards with 10-12 prestained proteins. They are engineered so that they produce sharp, evenly-spaced proteins prestained with different colors, making it easier and simpler to estimate the approximate molecular weight of proteins. Prestained recombinant protein ladders: These are highly preferred ladders for research use.Researchers typically do not prefer these because they tend to produce indistinct broad bands. Prestained naturally-occurring protein ladders: These produce broad bands when stained with one or more colors and show variability in the amount and location of dyes binding to each protein.Prestained protein ladders typically come in two types: These bands make it easier to monitor the protein migration patterns and calculate the molecular weights of proteins. Prestained protein ladders have three highly-intensified colored reference bands of 25 kDa, 50 kDa, and 75 kDa (kilodaltons). They can have either all proteins prestained with one color, two or more proteins prestained in different colors, or all proteins prestained with different colors. Prestained protein ladders, as the name suggests, have protein mixtures prestained with colors. Common Types of Protein Ladder Prestained Protein Ladders Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.Figure: A picture of protein electrophoresis with protein ladders in the first column of the immunoblot. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. Product Details Technical Data Sheet (pdf)įor research use only. Supplied in the sample loading buffer and is ready to use.Better performance for high molecular weight proteins.Comparable MW detection with competitor’s protein ladders.Suitable for size detection during protein purification.Excellent transfer efficiency on PVDF and Nitrocellulose membrane for WB.Clear separation and sharp bands in different SDS-PAGE gel and buffer systems.The Prime-Step™ Prestained Broad Range Protein Ladder is designed for monitoring protein separation during SDS-PAGE electrophoresis, confirming Western blot transfer quality on membranes (PVDF or nitrocellulose membrane) and for approximating the size of target proteins on the gel or blot. The Prime-Step™ Prestained Broad Range Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. It has 7 blue-stained bands and 3 reference bands (2 orange bands at 30 kDa and 270 kDa and 1 green band at 52 kDa) when separated on Tris-glycine gel. Lane 1: Prime-Step™ Prestained Broad Range Protein Ladder Lane 2: Competitor 1 protein ladder Lane 3: Competitor 2 protein ladder. Proteins were either stained with Coomassie blue, or transferred to nitrocellulose membrane followed by Ponceau red staining. Then protein ladders and total cell lysates were transferred to nitrocellulose (NC) and PVDF membrane followed by Ponceau red staining. Lane 1: Hela Lane 2: Jurkat Lane 3: NIH3T3 cells.ģµl Prime-Step™ Prestained Broad Range Protein Ladder (lane M) and 2µg recombinant proteins 1, 2, 3, with different molecular weights, was resolved by electrophoresis in 12% Bis-Tris gel (MES running buffer) followed by Coomassie blue staining.ģµl Prime-Step™ Prestained Broad Range Protein Ladder was resolved by electrophoresis in different gel running systems: 4-20% Tris-Glycine gel, 4-12% Bis-Tris gel (MES running buffer). Mw: molecular weight.ģµl Prime-Step™ Prestained Broad Range Protein Ladder (lane M) and total cell lysates (15µg total protein) was resolved by electrophoresis in 4-12% Bis-Tris gel (MOPS running buffer). Then protein ladders were transferred to nitrocellulose membrane. 3µl Prime-Step™ Prestained Broad Range Protein Ladder was resolved by electrophoresis in different gel running systems, including 14%, 4-20% and 4-12% Tris-Glycine gel, 4-12% Bis-Tris gel (MOPS and MES running buffer), 3-8% Tris-Acetate gel and 10-20% Tricine gel.
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